P-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles

P-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles

Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...

P-88: Expression Pattern of Maturation Genes During In Vitro Culture of Alginate Encapsulated Preantral Follicles Derived From Frozen-Thawed Mouse Ovaries

Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...

P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

Effect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles

P-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation

Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...